UNIQUE SERIES AKTU Syllabus
BBT653

Bioinformatics II Lab

L-T-P0-0-2 Credits1 Session2024-25 TypeLab / Practical
Appears in 1 semester

B.Tech Biotechnology · Sem 6 (Lab)

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Pre-requisites: Bioinformatics II theory course

List of Experiments / Activities

  1. 1Identification of Distantly related homologous sequences of a given query protein sequence using PSI-BLAST
  2. 2Construct Phylogenetic tree of five evolutionary related protein/nucleotide sequences
  3. 3Prediction of secondary structure of RNA using any web server.
  4. 4Construction and analysis of Ramachandran Plot using any suitable web server
  5. 5Align two homologous protein structure and calculation the RMSD for the superposition result
  6. 6Comparative assessment of best available tools for genome annotation
  7. 7Construction of restriction maps for various vectors used in genetic engineering using tool “NEB cutter”.
  8. 8Primer Design Construct primers for the given DNA sequence using any suitable web based tool
  9. 9Generate 2D QSAR model of a set of legend descriptor data
Course Objectives2 points
  • To introduce the fundamental principles of bioinformatics
  • To make them correlate theory and practical processes through experimentation.
Course Outcomes (COs)6 outcomes

After completion of the course student will be able to:

  1. CO1Understand the basic software and tools used in structure prediction of biomolecules
  2. CO2Conduct experimental procedure for Ramachandran plot and its analysis
  3. CO3Construct and analyse of restriction maps, QSAR model and homology model
  4. CO4Identify and structurally modify a natural product, to design a compound with the desired properties and to assess its therapeutic effects, theoretically.
  5. CO5Enhance their practical knowledge and thus their employability
  6. CO6Construction of primer for PCR.

Reference Book

  1. S. No.

Books

  1. Year of Publication/ Reprint
  2. Alphey L. DNA sequencing: from experimental methods to bioinformatics. BIOS scientific publishers Ltd; 1997. 1997
  3. Iftekhar M, Ghalib MR. Bioinformatics Practical Manual 2015
  4. Karthikeyan M, Vyas R. Practical chemoinformatics. Springer; 2014 May 6 2014
  5. Brown FK. Chemoinformatics: what is it and how does it impact drug discovery. Annual reports in medicinal chemistry. 1998 Jan 1;33:375-84 1998 B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow

Official AKTU syllabus

This subject is on page 54, 55 of the original AKTU PDF.

Official AKTU syllabus PDF ↗