UNIQUE SERIES AKTU Syllabus
BBT652

Plant Biotechnology Lab

L-T-P0-0-2 Credits1 Session2024-25 TypeLab / Practical
Appears in 1 semester

B.Tech Biotechnology · Sem 6 (Lab)

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Pre-requisites: Plant Biotechnology theory course

List of Experiments / Activities

  1. 1Preparation of Stocks solution for plant tissue culture media.
  2. 2Preparation of MS/B5 medium (semi-solid) and sterilization. B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow
  3. 3Explant selection, preparation and surface sterilization.
  4. 4To learn culturing, sub culturing and maintenance using selected explants.
  5. 5Initiation of in vitro cultures through axillary bud induction.
  6. 6Initiation of callus cultures from different explants.
  7. 7Preparation of artificial seed/synthetic seed for conservation of germplasm.
  8. 8Extraction of DNA/RNA from plants and its estimation.
  9. 9Isolation and characterization of plant secondary metabolites from selected medicinal plants. Extraction of proteins from plants and its estimation. B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow
Course Objectives3 points
  • To provide knowledge to apply fundamental principles of plant tissue culture.
  • To teach concepts behind culturing techniques from different explants.
  • To inculcate the hands on practice attitude in students to perform explants selection, media preparation, sterilization and callus culture initiation.
Course Outcomes (COs)6 outcomes

After completion of the course student will be able to:

  1. CO1Operate and handle the plant biotechnology lab equipment.
  2. CO2Perform tissue culture media preparation, sterilization and explants selection.
  3. CO3Understand in vitro cultures through axillary bud induction
  4. CO4Analyze plant secondary metabolites from selected medicinal
  5. CO5Learn DNA/RNA extraction and estimation
  6. CO6Perform extraction of plant proteins along with estimation.

Reference Book

  1. S. No.

Books

  1. Year of Publication/ Reprint
  2. Plant Biotechnology: Practical Manual by C.C. Giri, ArchanaGiri I. K. International Publications. 2007
  3. A Practical Manual For Plant Biotechnology by Tejovathi G, CBS Publishers and Distributors. 1996
  4. Plant Biotechnology: Laboratory Manual For Plant Biotechnology by H.S. Chawla, Oxford and IBH Publishing 2004

Official AKTU syllabus

This subject is on page 52, 53, 54 of the original AKTU PDF.

Official AKTU syllabus PDF ↗