UNIQUE SERIES AKTU Syllabus
BBT651

Bioprocess Engineering II Lab

L-T-P0-0-2 Credits1 Session2024-25 TypeLab / Practical
Appears in 1 semester

B.Tech Biotechnology · Sem 6 (Lab)

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List of Experiments / Activities

  1. II Lab EXAM DURATION: 2 Hours SEMESTER: VI (EVEN) L: T: P :: 0 : 0 : 2 CREDITS: 1
  2. 1Estimation and separation of proteins using different techniques.
  3. 2Bacterial cell disruption using different methods like physical, chemical and enzymatic methods.
  4. 3Downstream processing of a recombinant protein over expressed in bacterial system.
  5. 4Separation of a recombinant protein in bacterial cell lysate using Ammonium sulphate precipitation.
  6. 5High resolution purification of the recombinant protein by affinity chromatography.
  7. 6Downstream processing of lysozyme from egg white using ion exchange chromatography.
  8. 7Downstream processing of lysozyme from egg white using ion exchange chromatography.
  9. 8Downstream processing of caffeine from tea dust.
  10. 9Experiments involving crystallization of the downstream processed material. Product polishing by lyophilisation and drying. B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow
Course Objectives2 points
  • To impart knowledge about the basic fundamental principles of bioprocess engineering by performing different experiments.
  • To make them correlate theory and practical process by experimentation.
Course Outcomes (COs)6 outcomes

After completion of the course student will be able to:

  1. CO1Learn process of cellular disruption and cell components separation
  2. CO2Learn estimation and separation of proteins using different techniques.
  3. CO3Understand the process of manufacturing and processing of recombinant proteins.
  4. CO4Understand the basics of different chromatographic techniques and their applications in manufacturing industries.
  5. CO5Learn the process of isolation and extraction of phytochemicals
  6. CO6Learn product polishing techniques.

Reference Book

  1. S. No.

Books

  1. Year of Publication/ Reprint
  2. Scopes, R. K. Protein Purification: Principles and Practice, 3rd Edn, Springer. 2013
  3. Andreas, H., Walker, J. M., Wilson, K., Clokie, S. (Eds.). Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, United Kingdom: Cambridge University Press. 2018
  4. Belter, P. A., Cussler, E. L., and Hu, W.S. Bioseparation: Downstream Processing for Biotechnology, 2nd Edn., Wiley-Interscience. 1994 B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow
  5. Abelson, J. N. Simon, M. I. and Deutscher, M. P. Methods in Enzymology: Guide to Protein Purification, Volume 182, Academic Press. 1990
  6. Published research articles related to the experiments

Official AKTU syllabus

This subject is on page 50, 51, 52 of the original AKTU PDF.

Official AKTU syllabus PDF ↗