Plant Biotechnology

Laboratory organization; Principles of Plant Tissue Culture. Concepts of totipotency, explants, inoculums, acclimatization. Nutrition of plant cells; Nutrient media: Composition of commonly used nutrient culture media with respect to their contents like inorganic chemicals, organic constituents. An appraisal of different media, selection of media, Sterilization of the media. Hormones: Auxins, Cytokinins, Gibberellins, Abscisic Acid, Ethylene etc. Explant preparation and Surface sterilization. Basic procedure for Aseptic Tissue transfer.

Culture of plant materials- explants selection and technique of culturing. Organogenesis, Embryogenesis, Somaclonal variation, germiclonal variation. Establishment, growth and maintenance of Callus and cell suspension culture, Methods of sub culturing and transfer of regenerated plants to the field. Tissue and organ culture; Cellular differentiation and regulation of morphogenesis; Somatic embryogenesis; Control of organogenesis and embryogenesis; Single cell culture

Androgenesis; Anther and microspore culture; Gynogenesis; Embryo culture and rescue in agricultural and horticultural corps; Protoplast isolation; Culture– regeneration; Somatic hybrid-cybrids; In vitro selection of mutants – mutants for salts, disease, cold, drought, herbicide and other stress conditions; Micropropagation: Application of micropropagation in agriculture and forestry. Meristem culture and virus elimination; Shoot tip culture.

Improved crop varieties through somaclonal variation in invitro cultures. Application of tissue culture for crop improvement in agriculture, horticulture and forestry. Cryopreservation and slow growth cultures, Freezing and storage, thawing, reculture. Application of plant tissue culture production of secondary metabolites and other industrial products.

Genetic transformation using Ti plasmid Manipulation of gene expression in plants; Production of marker free transgenic plants. Developing insect-resistance, diseaseresistance, herbicide resistance plants. Genetic manipulation of flower pigmentation, Developing quality of seed storage, Provitamin A, iron proteins in rice, modification of food plant taste and appearance, yield increase in plants. B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow