Bioprocess Engineering II

Material and Energy Balance Introduction to conversion of units, Dimensionless groups applied in bioprocess, graphical differentiation and integration, material balance equation for steady and unsteady state, simplifications for steady-state processes with and without chemical reaction, stoichiometry of cell growth and product formation, degrees of reduction of substrate and biomass, yield coefficients of biomass and product formation, maintenance coefficients, oxygen consumption and heat evolution in aerobic cultures. Energy balance - heat capacity, estimation of heat capacities, general energy balance, Enthalpy calculation, enthalpy change: heat of combustion, heat of reaction for biomass production processes, energy-balance equation for cell culture and fermentation processes.

classification of reactions, reaction rate, speed of reaction, rate equation, concentration-dependent term of rate equation, rate constant, order and molecularity, representation of elementary and non-elementary reactions, Kinetic models for non-elementary reactions, temperature dependent term of a rate equation, activation energy and temperature dependency.

Integral and differential methods for analyzing kinetic data, interpretation of constant volume batch reactor, data for zero, first, second and third order reactions, half-life period, irreversible reaction in parallel and series, auto catalytic reactions. Kinetic interpretation of batch reactor data for single reactions, interpretation of variable volume batch reaction data for zero, first, second and third order reactions, Ideal batch reactor, steady state CSTR and plug flow reactors and their use for kinetic interpretation. Design for single reaction: size comparison of single reactors, plug flow reaction in series and/or parallel, equal and different size of mixed reactor in series, finding the best system B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow of given conversion, recycle reactor, Design of multiple reactions in batch, CSTR and PFR.

Downstream Processing Introduction to the various downstream processing steps and their significance in biotechnology, Cell disruption; cell separation, Centrifugation and Filtration: Flocculation, and sedimentation. Extraction: Basic equations of extraction, Aqueous two-phase extraction, batch extraction, staged extraction and differential extraction, Supercritical fluid extraction; Adsorption and Leaching, Precipitation; Membrane-based purification: Reverse osmosis, Dialysis.

Product Resolution / Fractionation and Polishing General chromatography theory and the different chromatographic techniques like adsorption, partition, ion exchange, affinity, gel filtration and HPLC, Dialysis, Crystallization, and Drying. Any Emerging Technologies in downstream processing in biotechnology industries. B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow