UNIQUE SERIES AKTU Syllabus
BBT061

Animal Biotechnology

L-T-P3-0-0 Credits3 Session2024-25 TypeTheory
Appears in 1 semester

B.Tech Biotechnology · Sem 6 (Departmental Elective-III)

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Pre-requisites: Basic Knowledge of

Syllabus

5 units · 40 lecture hours
Unit1

Unit 1

8 hrs

Basic cell culture techniques, Types of cell culture media; Ingredients of media; Physiochemical properties; CO2 and bicarbonates; Buffering; Oxygen; Osmolarity; Temperature; Surface tension and foaming; Balance salt solutions; Antibiotics growth supplements; Foetal bovine serum; Serum free media; Trypsin solution; Selection of medium and serum; Conditioned media; Other cell culture reagents; Preparation and sterilization of cell culture media, serum and other reagents.

Unit2

Unit 2

8 hrs

Different tissue culture techniques; Types of primary culture; Chicken embryo fibroblast culture; Chicken liver and kidney culture; Secondary culture; Trypsinization; Cell separation; Continuous cell lines; Suspension culture; Organ culture etc.; Behaviour of cells in culture conditions: division, growth pattern, metabolism of estimation of cell number; Development of cell lines; Characterization and maintenance of cell lines, stem cells; Cryopreservation; Common cell culture contaminants

Unit3

Unit 3

8 hrs

Cell cloning and selection; Transfection and transformation of cells; Commercial scale production of animal cells, stem cells and their application; Application of animal cell culture for in vitro testing of drugs; Testing of toxicity of environmental pollutants in cell culture; Application of cell culture technology in production of human and animal viral vaccines and pharmaceutical proteins

Unit4

Unit 4

8 hrs

Cell culture reactors; Scale-up in suspension; Scale and complexity; Mixing and aeration; Rotating chambers; Perfused suspension cultures; Fluidized bed reactors for suspension culture; Scale-up in monolayers; Multi-surface propagators; Multi-array disks, spirals and tubes; Roller culture; Micro carriers; Perfused monolayer cultures; Membrane perfusion; Hollow fibre perfusion; Matrix perfusion; Microencapsulation; Growth monitoring

Unit5

Unit 5

8 hrs

Transgenic animal production; Methods of transgene delivery; Integration of foreign genes and their validation; Gene targeting; Methods and strategies; Improving transgene integration efficiency; Cell lineages and developmental control genes in drosophila and mice; Differentiation of germ layers; Cellular polarity; Stem cell differentiation; Blood cell formation; Fibroblasts and their differentiation; Differentiation of cancerous cells and role of proto oncogenes B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow

Course Objectives3 points
  • To introduce in vitro culture techniques of animal cells and tissues
  • To learn different types of culture systems and reactors used for culturing of animal cells
  • To elaborate various applications of animal tissue cultures with specific reference to transgenic animal production
Course Outcomes (COs)6 outcomes

After completion of the course student will be able to:

  1. CO1Understand basics of animal tissue culture and its importance
  2. CO2Learn about the different types of animal cell culture media, their maintenance and cultivation of cell lines.
  3. CO3Understand techniques to establish animal cell cultures invitro as well as different types of reactors and their working
  4. CO4Learn the strategies involved in developing clones in lab
  5. CO5Understand the methods of transgene delivery and production of transgenic animals
  6. CO6Understand the process of stem cell differentiation and their applications with case studies

Reference Book

  1. S. No.

Books

  1. Year of Publication/ Reprint
  2. B. Hafez and E.S.E Hafez, Reproduction in farm animals, 7th Edition, Wiley Blackwell 2000
  3. G.E. Seidel, Jr. and S.M. Seidel, Training manual for embryo transfer in cattle (FAO Animal Production and Health Paper-77), 1st Edition, W.D. Hoard and sons FAO 1991
  4. I. Gordon, Laboratory production of cattle embryos, 2nd edition, CAB International 2003
  5. I. Gordon, Laboratory production of cattle embryos, 2nd edition, CAB International 1997 B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow
  6. Animal cell culture: Ian Freshney 2015

Official AKTU syllabus

This subject is on page 41, 42, 43 of the original AKTU PDF.

Official AKTU syllabus PDF ↗