UNIQUE SERIES AKTU Syllabus
BBT551

Genetic Engineering Lab

L-T-P0-0-2 Credits1 Session2024-25 TypeLab / Practical
Appears in 1 semester

B.Tech Biotechnology · Sem 5 (Lab)

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List of Experiments / Activities

  1. 2 EXAM DURATION: 2 HOURS SEMESTER: V
  2. 3L:T:P:: 0 : 0 : 2 CREIDTS : 1
Course Objectives1 points
  • To isolate the various biomolecules and genetic materials from cells and tissues To develop understanding of estimation of Genetic material To provide practical knowledge restriction digestion, transformation, screening and verification of cloning Practical knowledge of ligation, blotting and cloning.
Course Outcomes (COs)6 outcomes

After completion of the course student will be able to:

  1. CO1Demonstrate the isolation genetic materials
  2. CO2Perform experiments related to cloning, ligation, restriction digestion and transformation etc.
  3. CO3Demonstrate the Southern Blotting for identification of desired DNA in a pool DNA samples
  4. CO4Perform the bacterial cell competent for transformation
  5. CO5To be able to express foreign protein
  6. CO6To be able to use acquired knowledge for commercial products

REFERENCE BOOKS

  1. Laboratory manual on Molecular Biology & genetic Engineering-A new approach (2012), R.S. Sengar
  2. Laboratory Manual for Genetic Engineering (2009) S. john Vennison. Prentics hall publication B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow COURSE DETAILS: Genetic Engineering Lab S. NO. LIST OF EXPERIMENT
  3. Isolation of RNA and its estimation by orcinol method
  4. Isolation of plasmid DNA and its estimation by diphenylamine reaction
  5. Elution of plasmid DNA from agarose gel
  6. To perform restriction digestion of λ DNA
  7. Dephosphorylation of restriction enzyme digested vector pUC18
  8. To make bacterial cells competent for transformation
  9. To perform of transformation of the desired bacterial strain with plasmid DNA
  10. Screening of transformed colonies by X gal and IPTG
  11. Verification of cloning by colony PCR and screening of the positive colonies
  12. To perform a Southern Blotting for identification of desired DNA in a pool DNA samples
  13. To perform ligation of λ EcoRI digest using T4DNA ligase B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow

Official AKTU syllabus

This subject is on page 27, 28, 29 of the original AKTU PDF.

Official AKTU syllabus PDF ↗