UNIQUE SERIES AKTU Syllabus
BBT502

Fermentation Biotechnology

L-T-P3-1-0 Credits4 Session2024-25 TypeTheory
Appears in 1 semester

B.Tech Biotechnology · Sem 5 (Core)

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Pre-requisites: Basic Knowledge of elementary microbiology and basic bioprocessing

Syllabus

5 units · 35 lecture hours
Unit1

Unit 1: Introduction to fermentation technology

8 hrs

Interaction between Bio-chemical engineering, Microbiology and Biochemistry. History and development of fermentation industry: Microbial culture selection for fermentation processes, Strain development; Preservation and improvement of industrially important microorganisms.

Unit2

Unit 2: Inoculum development for industrial fermentation & Microbial Kinetics

9 hrs

Introduction, Criteria for transfer of inoculum, development of inocula for bacterial processes, yeast processes and mycelial processes. Inoculum development for plant fermenter, aseptic method of inoculation, achievement and maintenance of aseptic conditions. Fermentation Material and Energy balance, Microbial growth kinetics: Microbial growth cycle, measurement of growth, Batch culture, continuous culture, fed-batch culture, applications and examples.

Unit3

Unit 3

9 hrs

Media ingredients, medium formulation, oxygen requirements, antifoams, medium optimization, Media sterilization, Batch Process (thermal death kinetics), continuous sterilization process; sterilization of fermenter and other ancillaries, filter sterilization of air and media.

Unit4

Unit 4

8 hrs

Different regulatory mechanisms involved in controlling the catabolic and anabolic processes of microbes. Induction, nutritional repression, carbon catabolite repression, crabtree effect, feedback inhibition and feedback repression; Concept for overproduction ofprimary and secondary metabolites.

Unit5

Unit 5

1 hrs

Details of the process, parameters and materials -for the industrial manufacture of Antibiotics (β-lactum), Solvents (acetone) Amino acid (Lysine), Organic acids (Citric acid), Alcohols (Ethanol), Ind. Enzymes (Protease/Amylase) and Biopharmaceuticals (Insulin/Interferon etc.)- Microbial Transformations, Microbial leaching. B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow

Course Objectives1 points
  • o provide knowledge of fermentation technology and its industrial application. o teach the inoculums development, microbial kinetics and its measurement. o develop understanding of media component, sterilization and types of fermentation processes. o provide knowledge of regulation, control and overproduction of metabolites. o impart knowledge related to production and application of metabolites.
Course Outcomes (COs)6 outcomes

After completion of the course student will be able to:

  1. CO1nderstanding of the concepts and process technologies of fermentation.
  2. CO2pplication and use of different raw materials and its use in industrial scale production.
  3. CO3egulatory system in the microorganism.
  4. CO4rain improvement technologies and its role in Fermentation.
  5. CO5oncepts of the scale up and scale down criteria of fermentation process and production of metabolites.
  6. CO6Concepts about the processing and industrial manufacturing of antibiotics.

REFERENCE BOOK

  1. O Levenspeil (2006) Chemical Reaction Engineering, 3rd Edition, Wiley India.
  2. D.W.Mount; Bioinformatics-Sequence and genome analysis; Cold Spring HarbourLab press.
  3. B.N.Mishra; Bioinformatics: Concept and application, Pearson Education (in press)
  4. O’ Reilly; Developing Bioinformatics computer skills-1stIndian edition, SPD publication.
  5. Anthony J.F. Griffiths et al; An introduction to genetic analysis, 1st Ed
  6. Michael Starkey and Ramnath Elaswarapu; Genomics protocols, Humana press B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow Course Details: Fermentation Biotechnology

Official AKTU syllabus

This subject is on page 7, 8, 9 of the original AKTU PDF.

Official AKTU syllabus PDF ↗