Note: AKTU published this part of the syllabus as a scanned image. The text below was read from that image and is being checked —
if anything looks wrong, please refer to the official AKTU PDF.
List of Experiments / Activities
- 1Production of commercially important enzymes from microbial sources.
- 2Isolation of alpha amylase from plant source
- 3Determination of enzyme activity and specific activity.
- 4Partial purification of isolated enzymes.
- 5Method of checking the purity of the enzyme -SDS-PAGE
- 6Characterization of enzymes-effect of pH , temperature and inhibitors on enzyme activity etc.
- 7Identification of Enzyme by different assay
- 8Purification of enzymes by different methods
- 9Immobilization of enzymes –Different Techniques such as adsorption, entrapment, encapsulation and crosslinking.
- 10Strain improvement techniques- physical, chemical and genetic manipulation methods.
- 11Formulation of enzyme stability.
- 12Enzyme inhibition
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Exhibitthe extraction of enzyme and its enzyme activityK2
- CO2Perform purification and characterization of enzymeK2
- CO3Identity enzyme by enzyme assaysK3
- CO4Perform enzyme immobilization and strain improvement for increased productionK2
- CO5Comprehend the concept of enzyme inhibition.K3
Text books
- “An Introduction to Practical Enzyme Engineering”, Tata McGraw-Hill. R. Eisenthal and M.J. Dansen, “Enzyme Assays –A Practical Approach”, IRL Press, Oxford University Press, Oxford, 1993
Official AKTU syllabus
This subject is on page 15 of the original AKTU PDF.