Note: AKTU published this part of the syllabus as a scanned image. The text below was read from that image and is being checked —
if anything looks wrong, please refer to the official AKTU PDF.
List of Experiments / Activities
- 1How to calculate genetics and allelic frequencies numeric problem analysis.
- 2Isolation of Plasmid DNA.
- 3Isolation of Plant DNA.
- 4Estimation of DNA content in the given sample by spectrophotometer.
- 5Determination of Tm of DNA.
- 6Isolation of bacterial genomic DNA.
- 7Purification of DNA through Electrophoresis & visualization under UV transilluminator.
- 8Polyacrylamide gel electrophoresis of DNA.
- 9PCR amplification of DNA and visualization by gel electrophoresis.
- 10Isolation and study of polytene chromosome in Drosophila.
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Calculate the allelic frequencies of numeric problems.K2
- CO2Isolate DNA from plant cell, plasmid DNA and bacterial genomic DNA from.K4
- CO3Determine the Tm of DNA and analyze the concentration of DNA in a given cell using spectrophotometer.K4
- CO4Purify the DNA using electrophoresis and its visualization under transilluminator.K4
- CO5Separate DNA using PAGE and amplification of DNA using polymerase chain reaction.K4
Text books
- Molecular Biology A Practical Manualby PVGK Sharma, 2021.
Official AKTU syllabus
This subject is on page 14 of the original AKTU PDF.