BBT451

Bioprocess Engineering lab

L-T-P0-0-2 Credits1 Session2023-24 TypeLab / Practical
Appears in 1 semester

B.Tech Biotechnology · Sem 4 (Lab)

Note: AKTU published this part of the syllabus as a scanned image. The text below was read from that image and is being checked — if anything looks wrong, please refer to the official AKTU PDF.

List of Experiments / Activities

  1. 1Determine the growth patterns and specific growth rate of E.coli.
  2. 2Determine the effect of peptone concentration on E.coligrowth .
  3. 3Determination of specific thermal death rate constant (Kd) for E.Coli.
  4. 4Determine the effects of temperature & pH on Psuedomonasputida
  5. 5Upstream and Downstream of bioprocess for the production of Citric acid by Aspergillusniger.
  6. 6Citric acid production from whey with glucose as supplementary carbon source by Aspergillusniger.
  7. 7Upstream and Downstream of bioprocess for the production of α amylase
  8. 8Estimation of volumetric liquid mass transfer coefficient (KLa) using sodium sulphite method.
  9. 9Preparation of immobilized enzymes & cells and evaluation of kinetic parameters.
  10. 10Computational Design of Fermentative Process for L-lysine production.
Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

  1. CO1Demonstrate the growth pattern and death kinetics of E. coli.K3
  2. CO2Discuss the upstream and downstream bioprocessing for product formationK3
  3. CO3Analyze the mass transfer concepts in bioprocess.K3
  4. CO4Perform immobilization of enzymes and microbial cell.K2
  5. CO5Develop computational design for fermentative production.K4

Text books

  1. Practical Manual on Fermentation Technology by S. Kulandaivelu, S. Janarthanan 2012
  2. J.Jayaraman , “Laboratory Manual in Biochemistry”, New Age International Publications 2007
  3. Eisenthal, R. &Danson N.J. (Eds) Enzyme Assays: “A Practical Approach”, IRI Press, Oxford, UK 1992.

Official AKTU syllabus

This subject is on page 13 of the original AKTU PDF.

Official AKTU syllabus PDF ↗