BBT353

Biochemistry lab

L-T-P0-0-2 Credits1 Session2023-24 TypeLab / Practical
Appears in 1 semester

B.Tech Biotechnology · Sem 3 (Lab)

Note: AKTU published this part of the syllabus as a scanned image. The text below was read from that image and is being checked — if anything looks wrong, please refer to the official AKTU PDF.

List of Experiments / Activities

  1. Preparation of solutions: (i) Percentage solutions, (ii) Molar solutions, (iii) Normal solutions
  2. 2Titration of weak acid-weak base
  3. 3To perform quantitative estimation of carbohydrates
  4. 4To distinguish reducing and non-reducing sugars
  5. 5To perform quantitative estimation of proteins
  6. 6To estimate nucleic acids
  7. 7To perform isoelectric precipitation
  8. 8To separate sugars, fatty acids and amino acids by paper chromatography
  9. 9To extract lipids from plant material
  10. 10To perform thin layer chromatography
Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

  1. CO1Understand the basics of molarity, normality, buffers and pH meter.K2
  2. CO2Demonstrate the basics of titration and carbohydrate estimation.K4
  3. CO3Exhibit the difference between reducing and non-reducing sugars and estimation of proteins and nucleic acids.K4
  4. CO4Perform the extraction method of lipids and proteins on the basis of isoelectric point.K4
  5. CO5Understand the significance of chromatography.K4

Text books

  1. Wilson and Walker, “Principles and Techniques of Practical Biochemistry”, 4 Edn., Cambridge Knew pros 1997.
  2. Plummer DT, “An Introduction to Practical Biochemistry”, III Edn., Tata McGraw hill.

Official AKTU syllabus

This subject is on page 9 of the original AKTU PDF.

Official AKTU syllabus PDF ↗