Note: AKTU published this part of the syllabus as a scanned image. The text below was read from that image and is being checked —
if anything looks wrong, please refer to the official AKTU PDF.
List of Experiments / Activities
- 1Preparation of nutrient agar slants, plates and nutrient broth and their sterilization. (Microwave Oven, Heating mantles, Fridge, Heating Oven, Tube racks)
- 2Inoculation of agar slants, agar plate and nutrient broth (Incubators, Water bath, Laminar hood, dry heat sterilizer i.e. bead sterilizer)
- 3Culture of microorganisms using various techniques. (Shakers i.e. Cooling and Open shaker).
- 4Simple and differential staining procedures, endospore staining, flageller staining, cell wallstaining, capsular staining, negative staining. (Moist chambers, spirit lamps, slides, loops & microscopes, haemocytometer)
- 5Bacterial colony counting. (Moist chambers, spirit lamps, slides, loops & microscopes, haemocytometer)
- 6Isolation of microbes from soil samples and determination of the number of colony forming units. (U.V. spectrophotometer, Colony counter etc.)
- 7To determine the blood group and Rh of given blood sample.
- 8To perform single radial immunodiffusionanddoubleimmunodiffusion
- 9To perform rocket immune electrophoresis
- 10To perform counter current immune electrophoresis
- 11To perform ELISA
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Apply different sterilization method and inoculation methods.K2
- CO2Demonstrate different staining procedures and culturing of microbes.K2
- CO3Exhibit different methodology for colony counting.K1
- CO4Understand the principle of blood grouping and immunodiffusion.K3
- CO5Apply ELISA and electrophoresis.K2
Text books
- Lab Manual in microbiology by P Gunasekaran (New Age Int. Pub.).
Official AKTU syllabus
This subject is on page 8 of the original AKTU PDF.