Note: AKTU published this part of the syllabus as a scanned image. The text below was read from that image and is being checked —
if anything looks wrong, please refer to the official AKTU PDF.
List of Experiments / Activities
- 1Demonstration of basic concept of precision and accuracy using appropriate experimental data
- 2Study of Beer-Lambert’s law-using UV-Visible spectrophotometer.
- 3To study principle and working of laboratory microscope.
- 4To analyze the isolated plant pigments using paper chromatography.
- 5Separation of amino acids using thin layer chromatography.
- 6Separation of a mixture of polar and non polar compounds using column chromatographic technique.
- 7To study and analysis of DNA sample by agarose gel electrophoresis.
- 8To study and analysis of protein sample by SDS- PAGE
- 9To study the separation of compounds using liquid-liquid extraction experiments.
- 10To study the separation of biological compounds using various membrane separation.
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Understand concept of precision, accuracy for principle and working of laboratory microscope.K2
- CO2Learn and apply the spectrophotometric techniques for identification or quantification of biomolecules.K2
- CO3Understand the principle and execute the different chromatographic techniques for separation of biomolecules.K3
- CO4Apply the electrophoresis for quantitative and qualitative analysis of biomoleculesK3
- CO5Demonstrate the extraction and separation of biomolecules.K2
Text books
- Wilson and Walker, “Principles and Techniques of Practical Biochemistry” 4 Edn., Cambridge Knew pros 1997.
- Biotechniques: Theory & Practice: Second Edition by SVS Rana, Rustogi Publications.
- Biochemical Methods of Analysis: Saroj Dua And Neera Garg: Narosa Publishing House, New Delhi.
- Bioanalytical Techniques : ML Srivastava; Narosa Publishing House, New Delhi.
Official AKTU syllabus
This subject is on page 7 of the original AKTU PDF.