UNIQUE SERIES AKTU Syllabus
BBT073

Bioseperation & Down Stream

L-T-P3-0-0 Credits3 Session2025-26 TypeTheory
Appears in 1 semester

B.Tech Biotechnology · Sem 7 (Departmental Elective-IV)

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Pre-requisites: This course is designed for undergraduates with reasonably strong background in biology.

Syllabus

5 units · 40 lecture hours
Unit1

Unit 1: INTRODUCTION TO BIOSEPARATION PROCESS

8 hrs

Role and importance of bioseparation in biotechnological processes: RIPP scheme, Problems and requirements of bioproducts purification - Properties of Biomolecules - Characteristics of fermentation broth - Biological activity, Analysis of purity-Process economics: Capital and operating cost analysis.

Unit2

Unit 2: REMOVAL OF INSOUBLES

8 hrs

Cell disruption methods for intracellular products: Physical, chemical and mechanical - Removal of insolubles: Biomass and particulate debris separation techniques - flocculation - sedimentation - centrifugation and filtration methods.

Unit3

Unit 3: ISOLATION OF PRODUCTS

8 hrs

Adsorption: Principles - Langumir Freundlich isotherms - Extraction: Basics- Batch and continuous, aqueous two-phase extraction - supercritical extraction - in situ product removal - Precipitation: Methods of precipitation with salts - organic solvents and polymers - Membrane based separations: Micro and ultra filtration - theory - design and configuration of membrane separation equipments and its applications.

Unit4

Unit 4: PURIFICATION OF BIOPRODUCTS

8 hrs

Basic principles of Chromatographic separations: GC-HPLC - gel permeation - ion- exchange - affinity - reverse phase and hydrophobic interaction chromatography - Electrophoretic separation techniques: capillary - isoelectric focusing-2D gel electrophoresis - Hybrid separation technologies: GC-MS and LC-MS.

Unit5

Unit 5: PRODUCT POLISHING

8 hrs

Crystallization: Principles-Nucleation- Crystal growth-Kinetics-Batch crystallizers: Scale-up and design, Drying: PrinciplesWater in biological solids- Heat and mass transfer- Drying equipments: description and operation-Vacuum shelf - rotary dryer-Freeze dryer-Spray dryer. Biomolecules of Commercial importance Ethanol, citric acid, lysine, steroids, penicillin, dextran, trehalose, subtilisin, chymosin, vitamin B12, hepatitis B vaccine, insulin, erythropoietin, monoclonal antibodies. Total lectures

Course Objectives2 points
  • The major objective of this course is to impart in students the skills to operate bioprocesses for production of various Bio-products
  • This course is formulated to teach various methods of product separation, isolation and purification To teach the construction of genomic c-DNA libraries, cloning and strain improvement
Course Outcomes (COs)2 outcomes

After completion of the course student will be able to:

  1. CO1To operate, design and optimize the production medium, they will gain the ability to handle bioreactors to carry out different separation processes involving various types of bioproducts.Understand, Analyze
  2. CO2The students will be skilled in choosing a process of separation for a particular product, they will know how to design the relevant equipment, calculate the yield, and degree of purification.Understand, Analyze, Create

Note: The AKTU syllabus pages print this syllabus under code BBT072; the course structure table gives BBT073. The table code is shown here.

Reference Books

  1. S.No

Books

  1. YEAR OF PUBLICATION
  2. Raja Ghosh, “Principles of Bioseparations Engineering”, World Scientific Publishing 2006
  3. Ladisch. M. R, "Bioseparation Engineering: Principles, Practice and Economics", John Wiley & sons, New York 2001
  4. Asenjo.J.M, "Separation processes in Biotechnology" Marcel Dekker Inc 1993
  5. Bailey & oils, Biochemical Engg. Fundamentals, McGraw-Hill 1990
  6. Roger G. Harrison, Paul Todd, Scott R. Rudge, Demetri P. Petrides, "Bioseparation science and Engineering" Oxford University press. 2003

Official AKTU syllabus

This subject is on page 7, 8 of the original AKTU PDF.

Official AKTU syllabus PDF ↗