UNIQUE SERIES AKTU Syllabus
BBT072

Introduction to Stem cell technology

L-T-P3-0-0 Credits3 Session2025-26 TypeTheory
Appears in 1 semester

B.Tech Biotechnology · Sem 7 (Departmental Elective-IV)

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Pre-requisites: This course is designed for undergraduates with reasonably strong background in biology.

Syllabus

5 units · 48 lecture hours
Unit1

Unit 1

10 hrs

Introduction to Stem Cells: Properties, Classification and Sources Embryonic Stem Cells: Blastocyst and inner cell mass cells; Early embryogenesis; Organogenesis; Mammalian Nuclear Transfer Technology Adult Stem Cells: Stem cell differentiation, potency and aging, Nuclear reprogramming and gene editing Stem cells, Checkpoints and signaling in stem cells, Cryopreservation: Basic techniques, Cryoinjury, Cord blood cells cryopreservation

Unit2

Unit 2

10 hrs

Clinical Applications of Stem cells: Immune barriers of stem cell therapy, Engineering stem cell niche, Neurological disorders: Parkinson’s, Alzheimer’s, Huntington’s, Amyotrophic Lateral Sclerosis, Spinal Code Injury; Tissue systems failures: Organ failure, Diabetes, Cardiomyopathy, Hemophilia, Cancer Tissue Engineering: Introduction, Biomaterials, Biomaterial-tissue interface, Micro-scale technologies, Tissue repair and regeneration, Clinical applications and case studies

Unit3

Unit 3

8 hrs

Stem Cells in Tissue Engineering and Regenerative Medicine Applications in cartilage, bone, neural, and cardiac tissue regeneration, Integration of stem cells with scaffolds and bioreactors, Role of growth factors and signaling pathways in directing stem cell fate

Unit4

Unit 4

10 hrs

Primordial germ cells as stem cells, embryonal carcinoma cells as embryonic stem cells, trophoblast stem cells. Hematopoietic Stem Cells; Repopulating patterns of primitive hematopoietic stem cells, molecular diversification and developmental interrelationships, lymphopoiesis and the problem of commitment versus plasticity, hemangioblast, mesenchymal stem cells of human adult bone marrow, stem cells and neurogenesis. Epidermal Stem Cells: Liver stem cells, pancreatic stem cells, stem cells in the epithelium of the small intestine and colon.

Unit5

Unit 5

10 hrs

Biomedical applications of stem cells: Stem cell applications in understanding human developmental biology, high throughput drug screening and discovery, disease modeling and regenerative medicine; Genetically engineered stem cells; Current stem cell based clinical trials; Stem cells research ethics and guidelines; Controversies and ethical considerations, Stem cell research guidelines; Current challenges in clinical applications. Total lectures 48

Course Objectives1 points
  • This course is designed for students to provide an introduction to the role of stem cells in tissue growth and repair, the stem cells based therapy and in vitro construction of tissue and organ replacements, and the ethics and regulations in stem cells applications and research. By studying this course students should be able to supplement their future job/research endeavors with the subject’s knowledge.
Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

  1. CO1Understand the techniques used for the isolation, culture and manipulation of stem cells for use in disease therapy.Understand
  2. CO2Demonstrate the knowledge of adult cell reprogramming for producing stem cells for clinical use & Analyze and discuss the safety, ethical and legal considerations in human stem cell research and therapy.Understand, Analyze
  3. CO3Demonstrate comprehensive knowledge of stem cell biology, including their self-renewal, differentiation, and therapeutic potential & Apply advanced techniques for the isolation, culture, and genetic engineering of stem cells.Create
  4. CO4Integrate stem cells with biomaterials and bioreactors for tissue engineering and regenerative medicine applications.Create
  5. CO5Analyze and address clinical, ethical, and regulatory challenges in the translation of stem cell research into therapiesAnalyze

Note: The AKTU syllabus pages print this syllabus under code BBT073; the course structure table gives BBT072. The table code is shown here.

Reference Books

  1. S.No

Books

  1. YEAR OF PUBLICATION
  2. Stewart Sell, Stem Cells Handbook, Humana Press; Oct. 2003 2003
  3. Daniel R. Marshak, Richard L. Gardner and David Gottlieb, Stem Cell Biology, Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY, USA; 2001 2001
  4. Stem Cells and the Future of Regenerative Medicine, Committee on the Biological and Biomedical Applications of Stem Cell Research, Board on Life Sciences, National Academies Press 2002
  5. A.A. Vertes, N. Qureshi, A.I. Caplan, L. Babiss. Stem Cells in Regenerative Medicine: Science, Regulation and Business Strategies. Wiley-Blackwell. 2015
  6. Gabriela Rodrigues, Bernard A. J. Roelen, Concepts and Applications of Stem Cell Biology: A Guide for Students, Springer; 1st Edition. 2020
  7. R. Lanza and A. Atala. Essentials of Stem Cell Biology (3rd Ed.). Academic Press. 2013
  8. Song Li, Nicolas L’Heureux, Jenniffer Ellisseeff, Stem cell and Tissue Engineering, , WorldScientific 2011
  9. Gerhard M. Artmann, Stephen Minger, Jürgen Heschele, Stem Cell Engineering: Principles and Applications, Springer 2010
  10. Insoo Hyun, Bioethics and the Future of Stem Cell Research, Cambridge University Press 2013
  11. Mary Clarke, Jonathan Frampton, Stem Cells Biology and Application, Garland Science, 1st Edition 2020

Official AKTU syllabus

This subject is on page 9, 10 of the original AKTU PDF.

Official AKTU syllabus PDF ↗