Genomics and Proteomics

Origin of genomes, Acquisition of new genes, DNA sequencing – chemical and enzymatic methods, The origins of introns, Genetics to genomics to functional genomics. Forward genetics (Phenotype to gene structure) and Reverse genetics (Gene structure to phenotype). Chromosome structure and Genome organization, Genome sequencing methods, Genome assembly, Gene identification methods, Sequences Comparison Techniques, Genome annotation techniques.

Introduction to molecular markers-RFLP, RAPD, AFLP, SSRs and others. Genetic and physical maps, map based cloning, mapping population, southern and in situ hybridization for genome analysis, DNA fingerprinting; Single nucleotide polymorphisms, RNA interference, antisense RNA, siRNA, MiRNA, ; Human Genome Project; Pharmacogenomics: Ethical considerations of genetic testing; Genomics in drug discovery.

Proteomics Protein analysis (includes measurement of concentration, amino- acid composition, N-terminal sequencing); 2-D electrophoresis of proteins; Micro-scale solution iso-electric focusing; Peptide fingerprinting; LC/MS-MS for identification of proteins and modified proteins; MALDITOF; SAGE and Differential display proteomics, Yeast two hybrid system.

Microarray, Pharmacogenetics High throughput screening in genome for drug discovery - identification of gene targets, Pharmacogenetics and drug development.

Protein-protein and protein-DNA interactions; protein chips and functional proteomics; clinical and biomedical applications of proteomics; introduction to metabolomics, lipidomics, metagenomics and systems biology. Total lectures