UNIQUE SERIES AKTU Syllabus
BBT054

Metabolic Engineering

L-T-P3-0-0 Credits3 Session2024-25 TypeTheory
Appears in 1 semester

B.Tech Biotechnology · Sem 5 (Departmental Elective-I)

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Pre-requisites: Basic Knowledge of

Syllabus

5 units · 43 lecture hours
Unit1

Unit 1

6 hrs

Basic concept of metabolism, anabolism & catabolism, Importance of metabolic e ngineering, General Principles of Intermediary Metabolism, Regulation of Pathways, Strategies for Pathway Analysis. Understanding the role of Bioinformatics in the study of me tabolic pathways

Unit2

Unit 2: Synthesis of primary metabolites

8 hrs

Amino acid synthesis pathways and its regulation at enzyme level and whole cell level, Alteration of feedback regulation, Limiting accumulation of end products

Unit3

Unit 3: Biosynthesis of secondary metabolites

12 hrs

Regulation of secondary metabolite pathways, precursor effects, prophase, idiophase relationship, producers of secondary metabolites, applications of secondary metabolites.

Unit4

Unit 4: Bioconversions

7 hrs

Applications of Bioconversions, Factors affecting bioconversions, Specificity, Yields, Product inhibition, mixed or sequential bioconversions, Conversion of insoluble substances

Unit5

Unit 5: Regulation of enzyme production

10 hrs

Strain selection, Genetic improvement of strains, Gene dosage, metabolic pathway manipulations to improve fermentation, Feedback repression, Catabolite Repression, optimization and control of metabolic activities. B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow

Course Objectives1 points
  • To teach the concept and application of metabolic engineering To develop understanding metabolites production in different pathways and regulatory mechanism. To provide knowledge biosynthesis of metabolites To teach the bioconversions, product inhibition and factors affecting bioconversions.
Course Outcomes (COs)6 outcomes

After completion of the course student will be able to:

  1. CO1Explain basic concepts of metabolism and importance of metabolic engineering
  2. CO2Understand the production of metabolites and its regulatory mechanism
  3. CO3Explain the applications, specificity and product inhibition of bioconversion.
  4. CO4Regulation of enzyme production and strain improvement
  5. CO5Understand the general principles of intermediary metabolism, including the regulation of metabolic pathways at both the enzyme and cellular levels.
  6. CO6Explore mixed or sequential bioconversions and the conversion of insoluble substances.

REFERENCE BOOK

  1. G. Stephanopoulos, A. Aristidou and J. Nielsen, Metabolic Engineering Principles and Methodologies, Academic Press, 1998
  2. Daniel I. C. Wang, Malcolm D. Lilly, Arthur E. Humphrey, Peter Dunnill, Arnold l.Demain, Fermentation and Enzyme Technology,1st edition John Wiley& Sons, Reprint, 2005
  3. Christina Smolke, The Metabolic Pathway Engineering Handbook (Two Volume) Set 1st edition CRC press, 2009.
  4. Stanbury P. F. and Whitaker A., Principles of Fermentation Technology, Pergamon Press,1984. B.Tech. (Biotechnology) 3rd Year Dr. A.P.J. Abdul Kalam Technical University Uttar Pradesh, Lucknow COURSE DETAILS: METABOLIC ENGINEERING

Official AKTU syllabus

This subject is on page 17, 18, 19 of the original AKTU PDF.

Official AKTU syllabus PDF ↗