Vehicles for gene cloning - Biology of plasmids, Plasmid based vectors, Bacteriophage, Phage vectors, Cosmids, Phasmids, BAC and YAC vectors; Insert preparation- Restriction Digestion-Restriction enzymes, Polymerase chain reaction –Polymerase, Creation of restriction site by PCR.
Ligation –Ligase, cohesive, Blunt end ligation; Other Modification enzymes – Nucleases, Phasphatase, Kinase; Transformation-Preparation of competent cell, chemical and electrical transformation; Transfection; Recombinant selection and screening -Blotting technique.
Construction of cDNA library- construction subtractive cDNA library – construction of genomic DNA library – BAC library – YAC library.
Plasmid expression vectors-general features, promoters used in expression vectors -cloning of genes in correct reading frame in expression vector- purification of recombinant protein using Histidine tag, GST tag, chitin.
Applications of rDNA technology in Diagnostics and Therapeutics - HIV diagnosis, Human Insulin, Growth hormone; Recombinant Vaccines, Agricultural Technologies - Golden rice, Insect resistant crops, Genome Editing Tools using CRISPR Cas9 – Principle mechanism application ethics; Artificial Intelligence Application in RDT. Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh
After completion of the course student will be able to:
This subject is on page 5, 6, 7 of the original AKTU PDF.
