List of Experiments / Activities
- COMPUTATIONAL BIOLOGY LAB
- 1Knowledge of different biological database
- •Protein and gene sequence databases (NCBI, DDBJ, EMBL, SWISS PROT, PIR)
- •Structured at a bases (MMDB, PDB, CATH, SCOP)
- •Pathway Databases (KEGG, BRENDA, METACYC)
- •Bibliographic database (PUBMED, MEDLINE)
- 2Sequence retrieval from biological database
- 3To retrieve nucleotide sequences from GenBank in flat file format.
- 4To identify and analyze various fields (LOCUS, DEFINITION, ACCESSION, FEATURES, ORIGIN) in a GenBank flat file.
- 5Retrieve a protein structure (PDB file) from the Protein Data Bank (PDB) and visualize it using PyMOL, Chimera, or Jmol.
- 6Identify alpha-helices, beta-sheets, and active sites.
- 7Sequence similarity searching of nucleotide and protein sequences
- 8Finding homologous sequences
- 9Multiples Sequence alignment
- 10Dynamic programming method-local and global alignment
- 11Gene prediction methods
- Note: The instructor may add/delete/modify/tune experiments B. TECH (BIOINFORMATICS)
Course Outcomes (COs)
After completion of the course student will be able to:
- CO1Identify and describe different types of biological databases and their purposes.K1
- CO2Retrieve sequences from databases and perform similarity searches for nucleotide and protein data, study GenBank flat fileK3
- CO3Analyze protein properties and identify homologous sequences to infer functional/evolutionary relationships.K4
- CO4Perform multiple sequence alignment and apply dynamic programming methods for sequence comparison.K4
- CO5Evaluate and implement gene prediction methods to identify coding regions in genomic sequences.K5
Official AKTU syllabus
This subject is on page 9, 10 of the original AKTU PDF.