BBI352 Applied Microbiology and Immunology Lab Syllabus — AKTU 2025-26

List of Experiments / Activities

APPLIED MICROBIOLOGY AND IMMUNOLOGY LAB
1Sterilization techniques
2Media preparation
3Microscopy and Micrometry
4Serial dilution, Isolation of microbes, Pure culture techniques: streaking and spreading methods
5Staining Techniques (Simple, Gram staining) 6 Inoculation of agar slants, agar plate and nutrient broth
7Immunodiffusion
8Immunoelectrophoresis
9Plate ELISA
10Blood grouping
Note: The instructor may add/delete/modify/tune experiments; (*2 can be demonstration)

Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

CO1Understand the concept of Sterilization techniques,K3
CO2Understand the concept and learn to do Media preparation, Staining Techniques (Simple, Gram staining), Blood groupingK3
CO3Apply knowledge and technique for isolation of pure microbial cultures,K4
CO4Apply knowledge and technique to perform Immunodiffusion, Immunoelectrophoresis,K4
CO5Learn the principal of antigen antibody interaction and ELISA,K3

This subject is on page 8 of the original AKTU PDF.