BBI351

Basic Biochemistry Lab

L-T-P0-0-2 Credits1 Session2025-26 TypeLab / Practical
Appears in 1 semester

B.Tech Bioinformatics · Sem 3 (Lab)

List of Experiments / Activities

  1. BASIC BIOCHEMISTRY LAB
  2. 1Qualitative test of Carbohydrates
  3. 2Qualitative test of protein and amino-acids
  4. 3Quantitative Estimation of proteins
  5. 4Quantitative Estimation of glucose
  6. 5Paper chromatography
  7. 6To distinguish reducing sugar from non-reducing sugar
  8. 7To distinguish pentose from hexose
  9. 8To distinguish ketose from aldose
  10. 9To determine the presence of starch by iodine test
  11. 10To determine the presence of sucrose by acid base hydrolysis
  12. Note: The instructor may add/delete/modify/tune experiments; (*2 can be demonstration)
Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

  1. CO1Recall different carbohydrate tests and their principle.K1
  2. CO2Apply Lowry/Biuret method to determine protein concentration.K3
  3. CO3Analyze separation of amino acids/sugars based on Rf values, estimate concentration of biomoleculesK4
  4. CO4Understand principle of Benedict’s/Fehling’s test and interpret results.K2
  5. CO5Explain the iodine-starch complex formation and interpret the observed color change.K4

Official AKTU syllabus

This subject is on page 7 of the original AKTU PDF.

Official AKTU syllabus PDF ↗