BBI062

Bioprocess Engineering

L-T-P3-0-0 Credits3 Session2026-27 TypeTheory
Appears in 1 semester

B.Tech Bioinformatics · Sem 6 (Departmental Elective-III)

Syllabus

5 units · 40 lecture hours
Unit1

Unit 1: INTRODUCTION

8 hrs

Introduction to Bioprocess, Microbial process development, Fermentation Economics, Batch culture, Continuous culture, kinetics, Chemostat with recycle and multistage Chemostat Systems. Modify batch reactors: Fed-batch operation, Perfusion systems.

Unit2

Unit 2: BIOREACTOR DESIGN SCALE UP, SCALE DOWN

9 hrs

Bioreactor configurations, Analysis of ideal and non-ideal reactors. Multiphase reactors: packed-bed reactors, bubble-column bioreactors, fluidized bed bioreactors, trickle-bed reactors. Bioprocess Considerations: Animal cell cultures & plant cell cultures Scale up of bioprocesses-based on oxygen transfer, power consumption and impeller tip speed, Scale down.

Unit3

Unit 3: MIXING & MASS TRANSFER

8 hrs

Mixing equipments, flow patterns in reactors, mixing mechanism, power consumption and shear properties of sparged and agitated vessels and various mixing agitators. Diffusion, mass transfer, Oxygen transfer in fermenters.

Unit4

Unit 4: STERILIZATION, STRAIN IMPROVEMENT AND MEDIA FORMULATION

8 hrs

Design of batch and continuous sterilization processes, kinetics of thermal death of cells and spores. Strain improvement of industrially important microorganisms, Media formulation industrial fermentations.

Unit5

Unit 5: IMMOBILIZED CELL SYSTEMS (ICS)

7 hrs

Mass Analysis-Tandem Mass Spectrometry (MS/MS)-Ion Detectors-Ionization of the Sample. Sample Preparation/Analysis-Proteins and Peptides- Protein Folding. Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh

Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

  1. CO1Explain the principles of bioprocess engineering, microbial growth, and fermentation systems.K2
  2. CO2Apply bioreactor design concepts and scale-up criteria for industrial bioprocesses.K3
  3. CO3Explain mixing, mass transfer, and oxygen transfer phenomena in bioreactors.K2
  4. CO4Apply sterilization methods, strain improvement techniques, and media formulation strategies in fermentation processes.K3
  5. CO5Explain immobilized cell systems and proteomic techniques for modern bioprocess and biotechnology applications.K2

TEXT BOOKS

  1. / REFERENCES
  2. Pauline M. Doran, “Bioprocess Engineering Principles”, 8th ed., Academic Press, New York, 2003.
  3. M.L. Shuler and F. Kargi, “Bioprocess Engineering--basic Concepts”, 2nd Edn., Prentice-Hall Of India Pvt Ltd (2008).
  4. Peter F. Stanbury, Stephen J. Hall & A. Whitaker, “Principles of Fermentation Technology”, Elsevier India Pvt Ltd. (2007).
  5. Klaas Van’t Riet, Johannes Tramper, “Basic Bioreactor Design”, 2nd ed., Marcel Dekker, Inc., New York, 1991.
  6. Bailey and Ollis, “Biochemical Engineering Fundamentals”, 2nd ed., McGraw- Hill Book Company, New York, 1986.
  7. Mccabe L. Warren, Smith C. Julian and Peter Harriott, “Unit Operations of Chemical Engineering”, 6th ed., McGraw Hill International Edition, New York, 2001.
  8. Abhilasha S. Mathuriya, “Industrial Biotechnology”, 1st ed., Ane Books Pvt. Ltd., New Delhi, 2009. Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh

Official AKTU syllabus

This subject is on page 37, 38, 39 of the original AKTU PDF.

Official AKTU syllabus PDF ↗