UNIQUE SERIES AKTU Syllabus
BBI053

Metabolomics and Metabolic Engineering

L-T-P3-0-0 Credits3 Session2026-27 TypeTheory
Appears in 1 semester

B.Tech Bioinformatics · Sem 5 (Departmental Elective-I)

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Syllabus

5 units · 40 lecture hours
Unit1

Unit 1: INTRODUCTION TO METABOLOMICS

8 hrs

Role of metabolomics in systems biology –application of metabolomics- Analytical methods in metabolomics – Data standards– Databases for Chemical, Spectral and Biological Data –Reconstruction of dynamic metabolic network model- examples- study of metabolome of a simple organism like E. Coli.

Unit2

Unit 2: BIOINFORMATICS IN METABOLOMICS

8 hrs

Online databases and pipelines for metabolomics – GC-MS based metabolomics – Computational methods to compute and integrate metabolic data software for metabolomics- metabolomics and medical sciences.

Unit3

Unit 3: INTRODUCTION TO METABOLIC ENGINEERING

8 hrs

Importance of metabolic engineering- comprehensive models for cellular reactions-material balances & data consistency- metabolic pathway synthesis.

Unit4

Unit 4: METABOLIC FLUX ANALYSIS AND ITS APPLICATION

8 hrs

Theory determination of flux by isotope labeling-Metabolic control analysis- (control coefficients and summation theorems, FCC determination)-Grouping of reactions (gFCC, identification of independent pathways).

Unit5

Unit 5: METABOLIC NETWORKS

8 hrs

Kinetic model of metabolic networks Systems metabolic engineering of E. coli- bottom up and top down approaches of network analysis. Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh

Course Outcomes (COs)5 outcomes

After completion of the course student will be able to:

  1. CO1Understand the role of metabolomics, analytical methods, databases, and reconstruction of metabolic networks.K2
  2. CO2Apply bioinformatics tools and computational methods in metabolomics, including GC-MS pipelines and medical applications.K3
  3. CO3Apply metabolic engineering principles to construct metabolic pathways.K3
  4. CO4Apply metabolic flux analysis using isotope labeling, control analysis, and pathway grouping.K3
  5. CO5Explain metabolic networks, kinetic models, and systems metabolic engineering approaches.K2

TEXT BOOKS

  1. / REFERENCES
  2. Silas G. Villas-Boas, Jens Nielsen, Jorn Smedsgaard, Michael A. E. Hansen, Ute Roessner-Tunali, “Metabolome Analysis: An Introduction”, John Wiley & Sons, 2007.
  3. Jens Høiriis Nielsen, Michael C. Jewett, “Metabolomics: A Powerful Tool in Systems Biology”, Springer, 2007.
  4. Dr. Christoph Wittmann, Sang Yup Lee, “Systems Metabolic Engineering”, Springer, 2012.
  5. Gregory N. Stephanopoulos, “Metabolic Engineering- Principles and Methodologies”, Academic Press, First Edition, 1998.
  6. Ute Roessner, “Metabolomics”, InTech, 2012.
  7. Wolfram Weckwerth, “Metabolomics: Methods and Protocols”, Humana Press, 2007.
  8. Cortassa S., “An Introduction to Metabolic and Cellular Engineering”, World Scientific Publishing Company Ltd., 2002. Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh

Official AKTU syllabus

This subject is on page 15, 16, 17 of the original AKTU PDF.

Official AKTU syllabus PDF ↗